Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants (2024)

Yin, T., Lu, H. Y., Zhang, S. L., Liu, J. M., & Chen, D. M. (2009). Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants. Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji, 31(6), 663-667. https://doi.org/10.3724/SP.J.1005.2009.00663

Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants. / Yin, Tao; Lu, Hong Yu; Zhang, Shang Long et al.
In: Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji, Vol. 31, No. 6, 06.2009, p. 663-667.

Research output: Contribution to journal › Article › peer-review

Yin, T, Lu, HY, Zhang, SL, Liu, JM & Chen, DM 2009, 'Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants', Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji, vol. 31, no. 6, pp. 663-667. https://doi.org/10.3724/SP.J.1005.2009.00663

Yin T, Lu HY, Zhang SL, Liu JM, Chen DM. Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants. Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji. 2009 Jun;31(6):663-667. doi: 10.3724/SP.J.1005.2009.00663

Yin, Tao ; Lu, Hong Yu ; Zhang, Shang Long et al. / Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants. In: Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji. 2009 ; Vol. 31, No. 6. pp. 663-667.

@article{d5d4e5802a854e75b8ea33363c75651f,

title = "Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants",

abstract = "The AGPL1 (ADP-glucose pyrophosphorylase large subunit 1) promoter from watermelon (Citrullus vulgaris S.) has proved to exhibit fruit-specific expression patterns in tomato (Lycopersicon esculentum L.). A plant expression vector harboring sweet-taste protein, Brazzein, directed by AGPL1 promoter, was constructed and transferred into tomato plants through Agrobacterium-mediated transform methods. Histochemical staining assay, PCR screening, Southern blotting analysis and RT-PCR analysis showed that Brazzein gene was successfully integrated into the genome of transgenic tomato plants with stable expression. Sweet-taste fruits were produced under control of fruit-specific AGPL1 promoter, whereas other parameters of fruit quality were largely unchanged.",

author = "Tao Yin and Lu, {Hong Yu} and Zhang, {Shang Long} and Liu, {Jing Mei} and Chen, {Da Ming}",

year = "2009",

month = jun,

doi = "10.3724/SP.J.1005.2009.00663",

language = "English (US)",

volume = "31",

pages = "663--667",

journal = "Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji",

issn = "0253-9772",

publisher = "Ke xue chu ban she",

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T1 - Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants

AU - Yin, Tao

AU - Lu, Hong Yu

AU - Zhang, Shang Long

AU - Liu, Jing Mei

AU - Chen, Da Ming

PY - 2009/6

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N2 - The AGPL1 (ADP-glucose pyrophosphorylase large subunit 1) promoter from watermelon (Citrullus vulgaris S.) has proved to exhibit fruit-specific expression patterns in tomato (Lycopersicon esculentum L.). A plant expression vector harboring sweet-taste protein, Brazzein, directed by AGPL1 promoter, was constructed and transferred into tomato plants through Agrobacterium-mediated transform methods. Histochemical staining assay, PCR screening, Southern blotting analysis and RT-PCR analysis showed that Brazzein gene was successfully integrated into the genome of transgenic tomato plants with stable expression. Sweet-taste fruits were produced under control of fruit-specific AGPL1 promoter, whereas other parameters of fruit quality were largely unchanged.

AB - The AGPL1 (ADP-glucose pyrophosphorylase large subunit 1) promoter from watermelon (Citrullus vulgaris S.) has proved to exhibit fruit-specific expression patterns in tomato (Lycopersicon esculentum L.). A plant expression vector harboring sweet-taste protein, Brazzein, directed by AGPL1 promoter, was constructed and transferred into tomato plants through Agrobacterium-mediated transform methods. Histochemical staining assay, PCR screening, Southern blotting analysis and RT-PCR analysis showed that Brazzein gene was successfully integrated into the genome of transgenic tomato plants with stable expression. Sweet-taste fruits were produced under control of fruit-specific AGPL1 promoter, whereas other parameters of fruit quality were largely unchanged.

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